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Food Technology and Biotechnology, Vol. 56 No. 4, 2018.

Original scientific paper

https://doi.org/10.17113/ftb.56.04.18.5553

Alkaline and Halophilic Protease Production by Bacillus luteus H11 and Its Potential Industrial Applications

Agnieszka Kalwasińska orcid id orcid.org/0000-0002-2435-567X ; Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, PL-87100 Toruń, Poland
Urszula Jankiewicz orcid id orcid.org/0000-0003-2240-6629 ; Department of Biochemistry, Warsaw University of Life Sciences, Nowoursynowska 159, PL-02787 Warsaw, Poland
Tamás Felföldi orcid id orcid.org/0000-0003-2009-2478 ; Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/c, HU-1117 Budapest, Hungary
Aleksandra Burkowska-But orcid id orcid.org/0000-0002-7449-4678 ; Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, PL-87100 Toruń, Poland
Maria Swiontek Brzezinska ; Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, PL-87100 Toruń, Poland

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Abstract

This paper presents the results of the study on the production of protease by Bacillus luteus H11 isolated from an alkaline soda lime. B. luteus H11 was identified as an alkalohalophilic bacterium, and its extracellular serine endoprotease also showed an extreme alkali- and halotolerance. It was remarkably stable in the presence of NaCl up to 5 M. The enzyme was active in a broad range of pH values and temperatures, with an optimum pH of 10.5 and a temperature of 45 °C. It had a molecular mass of about 37 kDa and showed activity against azocasein and a synthetic substrate for the subtilisin-like protease, N succinyl-l-phenylalanine-p-nitroanilide. The halo-alkaline protease produced by B. luteus H11 seems to be significant from an industrial perspective because of its tolerance towards high salinity and alkalinity as well as its stability against some organic solvents, surfactants and oxidants. These properties make the protease suitable for applications in food, detergent and pharmaceutical industries, and also in environmental bioremediation.

Keywords

Bacillus; proteolytic bacteria; alkalohalophiles; serine endoprotease; subtilisins

Hrčak ID:

214964

URI

https://hrcak.srce.hr/214964

Publication date:

31.12.2018.

Article data in other languages: croatian

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