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Preliminary communication

https://doi.org/10.17113/ftb.57.01.19.5983

Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples

Konstantin A. Kurbakov ; V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Talalikhina 26, 109316, Moscow, Russian Federation
Evgenii A. Konorov ; Martsinovsky Institute of Medical Parasitology, Tropical and Vector- Borne Diseases, Sechenov University, Malaya Pirogovskaya 20-1, 119435 Moscow, Russian Federation
Mikhail Y. Minaev ; V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Talalikhina 26, 109316, Moscow, Russian Federation
Oksana A. Kuznetsova ; V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Talalikhina 26, 109316, Moscow, Russian Federation


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Abstract

Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair.

Keywords

real-time PCR; HRM; starter cultures; fermented sausages; Lactobacillus sakei; Lactobacillus curvatus

Hrčak ID:

218904

URI

https://hrcak.srce.hr/218904

Publication date:

29.3.2019.

Article data in other languages: croatian

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