Original scientific paper
Hydrolysis of lactose with -D-galactosidase
Vesna Stehlik-Tomas
; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Slobodan Grba
Damir Stanzer
Vlatka Gulan-Zetić
Abstract
The conditions of lactose hydrolysis with enzyme preparation of D-galactosidase were investigated. The aim of this work was to considered the use of whey in fermentative processes with yeast Saccharomyces cerevisiae. Enzymatic hydrolysis was conducted at different temperatures, with different lactose concentrations in medium and different concentrations of added enzyme. The results show that optimal temperature for hydrolysis was 40°C. The optimal amount of enzyme preparation was 2 gL-1 in lactose medium with 5-10 % lactose.
Keywords
Saccharomyces cerevisiae; whey; hidrolysis lactose
Hrčak ID:
1897
URI
Publication date:
12.6.2001.
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