Original scientific paper
https://doi.org/10.17113/ftb.58.03.20.6568
Evaluation of the Antidepressant Effect of the Functional Beverage Containing Active Peptides, Menthol and Eleutheroside, and Investigation of Its Mechanism of Action in Mice
Yuanjin Qi
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Huizhen Zhang
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Sha Liang
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Jiajia Chen
; Department of Food Science, University of Tennessee, 2510 River Dr, TN 37996 Knoxville, USA
Xiaoni Yan
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Zhouyu Duan
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Deyang Zhou
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Zhicheng Li
; College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China
Abstract
Research background. Depression has become a global threat to human health. In order to solve it, researchers have conducted multi-faceted studies including diet. Many food-derived bioactive substances have shown antidepressant effects. However, there are few studies on the design of industrialized food with antidepressant effect. This study aims to evaluate the antidepressant effect of afunctional beverage made from several ingredients with potential antidepressant function and investigate its antidepressant mechanisms.
Experimental approach. The beverage consists of peppermint oil, active peptides derived from bovine milk casein and Acanthopanax senticosus extract (ASE) whose active ingredient is eleutheroside. Different amounts of ASE were evaluated to determine the optimal concentration of eleutheroside in this functional beverage to deliver the best antidepressant effect through extensive behavioral testing, including preliminary acute stress
experiments and further chronic unpredictable mild stress test.
Results and conclusions. The results demonstrated that the beverage with 15 mg/kg of eleutheroside could significantly reduce the mice’s immobility time of tail suspension test and forced swimming test, recover mice’s sucrose preference and behavior changes in the open field test, improve the contents of dopamine, norepinephrine, 5-hydroxytryptamine and the activity of superoxide dismutase and reduce the content of malondialdehyde in mice’s brains, which indicated that the improvement of monoamine neurotransmitter systems and antioxidation was one potential mechanism of antidepressant action.
Novelty and scientific contribution. This study provides a design of antidepressant functional beverage and an efficient way for the prevention and treatment of depression.
Keywords
functional beverage; eleutheroside; behavioral testing; antidepressant mechanism; monoamine; antioxidation
Hrčak ID:
245679
URI
Publication date:
30.10.2020.
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