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Food Technology and Biotechnology, Vol. 46 No. 2, 2008.

Original scientific paper

Xylanase Production by Aspergillus niger LPB 326 in Solid-State Fermentation Using Statistical Experimental Designs

Giselle Maria Maciel ; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Luciana Porto de Souza Vandenberghe ; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Charles Windson Isidoro Haminiuk ; Food Technology Department, Federal University of Technology, Paraná (UTFPR), P.O. Box 271, Campo Mourão, PR, Brazil
Ricardo Cancio Fendrich ; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Bianca Eli Della Bianca ; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Tahiana Quintella da Silva Brandalize ; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Ashok Pandey ; Biotechnology Division, Regional Research Laboratory, CSIR, IN-695 019 Trivandrum, India
Carlos Ricardo Soccol ; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil

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Abstract

Xylanase was produced by Aspergillus niger LPB 326 cultivated on lignocellulosic substrate composed by sugarcane bagasse and soybean meal in solid-state fermentation. The effects of various variables were observed and optimized by applying statistical experimental designs. The best xylanase activity was obtained in a medium containing 10 g of sugarcane bagasse and soybean meal in the ratio of 65 and 35 %, respectively, moistened to 85 % of initial water content with a nutrient salt solution composed of (in g/L): CuSO4 0.4, KH2PO4 1.5 and CoSO4 0.0012, and incubated for 4 days at 30 °C. Under these optimized conditions, a xylanase activity of 3099 IU/g of dry matter was obtained.

Keywords

xylanase; Aspergillus niger; solid-state fermentation; statistical designs

Hrčak ID:

24407

URI

https://hrcak.srce.hr/24407

Publication date:

13.6.2008.

Article data in other languages: croatian

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