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ADMET and DMPK, Vol. 9 No. 4, 2021.

Original scientific paper

https://doi.org/10.5599/admet.1023

Synthesis of silymarin−selenium nanoparticle conjugate and examination of its biological activity in vitro

Sergey Staroverov ; Saratov State Agrarian University, Saratov, Russian Federation
Sergey Kozlov ; Saratov State Agrarian University, Saratov, Russian Federation
Alexander Fomin ; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation
Konstantin Gabalov ; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation
Vitaliy Khanadeev ; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation
Dmitry Soldatov ; Saratov State Agrarian University, Saratov, Russian Federation
Ivan Domnitsky ; Saratov State Agrarian University, Saratov, Russian Federation
Lev Dykman ; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation
Sergey V. Akchurin ; Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russian Federation
Olga Guliy orcid id orcid.org/0000-0002-0541-0020 ; Saratov State Agrarian University, Saratov, Russian Federation

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Abstract

Silymarin (Sil) was conjugated to selenium nanoparticles (SeNPs) to increase Sil bioavailability. The conjugates were monodisperse; the average diameter of the native SeNPs was ~ 20-50 ± 1.5 nm, whereas that of the conjugates was 30-50 ± 0.5 nm. The use of SeNPs to increase the bioavailability of Sil was examined with the MH-22a, EPNT-5, HeLa, Hep-2, and SPEV-2 cell lines. The EPNT-5 (glioblastoma) cells were the most sensitive to the conjugates compared to the conjugate-free control. The conjugates increased the activity of cellular dehydrogenases and promoted the penetration of Sil into the intracellular space. Possibly, SeNPs play the main part in Sil penetration of cells and Sil penetration is not associated with phagocytosis. Thus, SeNPs are promising for use as a Sil carrier and as protective antigens.

Keywords

selenium nanoparticles; silymarin, bioavailability; cell lines

Hrčak ID:

268287

URI

https://hrcak.srce.hr/268287

Publication date:

15.12.2021.

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