Original scientific paper

Medium Optimization for 5’-Phosphodiesterase Production from Penicillium citrinum Using Response Surface Methodology

Lu-E Shi ; College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, PR China
Guo-Qing Ying ; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, PR China
Xiao-Ying Zhang ; College of Pharmacy, South Dakota State University, Brookings, SD 57007, USA
Zhen-Xing Tang ; College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, PR China
Jian-Shu Chen ; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, PR China
Wen-Yue Xiong ; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, PR China
Hua-Zhang Liu ; College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, PR China

Abstract

Medium optimization for 5’-phosphodiesterase production from Penicillium citrinum was studied by applying one-factor-at-a-time method, orthogonal array method and response surface methodology in this paper. The one-factor-at-a-time method was used to study the effects of carbon, nitrogen, phosphorus and metal ion sources on 5’-phosphodiesterase production. Among various carbon and nitrogen sources used, glucose and peptone were the most suitable substances for 5’-phosphodiesterase production, respectively. Subsequently, the concentrations of glucose, peptone, groundnut meal, Zn2+ and KH2PO4 were optimized using the orthogonal array method. Response surface methodology was also applied for medium optimization. Glucose concentration (X1), peptone concentration (X2) and groundnut meal (X3) were selected as the independent variables. Results showed that the regression models adequately explained the data variation and represented the actual relationships between the parameters and responses. The optimum conditions were glucose at a fraction of 6.5 %, peptone at a fraction of 0.45 % and groundnut meal at a fraction of 1.0 %. Maximum enzyme activity was 353 U/mL under the optimum conditions. Maximum 5’-phosphodiesterase activity in media optimized by orthogonal method and response surface methodology was about 1.286 and 1.456 times, respectively, greater than in the medium optimized by one-factor-at-a-time method.

Keywords

medium optimization; 5’-phosphodiesterase; response surface methodology

Hrčak ID:

27129

URI

https://hrcak.srce.hr/27129

Publication date:

4.6.2007.

Article data in other languages: croatian

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