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Chemistry in Industry : Journal of Chemists and Chemical Engineers of Croatia, Vol. 72 No. 5-6 (special issue), 2023.

Original scientific paper

https://doi.org/10.15255/KUI.2022.077

HPLC Analysis of Phytosterols in Prunus spinosa L. Extracts and Their Antiproliferative Activity on Prostate Cancer Cell Lines

Alema Dedić orcid id orcid.org/0000-0003-1920-7645 ; University of Sarajevo, Faculty of Pharmacy, Department of Chemistry in Pharmacy, Zmaja od Bosne 8, Sarajevo, Bosnia and Herzegovina
Hurija Džudžević-Čančar ; University of Sarajevo, Faculty of Pharmacy, Department of Chemistry in Pharmacy, Zmaja od Bosne 8, Sarajevo, Bosnia and Herzegovina
Tatjana Stanojković ; Institute of Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia
Marin Roje orcid id orcid.org/0000-0002-7478-1490 ; Ruđer Bošković Institute, Department of Organic Chemistry and Biochemistry, Bijenička cesta 54, Zagreb, Croatia
Ana Damjanović ; Institute of Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia
Amra Alispahić orcid id orcid.org/0000-0001-8447-936X ; University of Sarajevo, Faculty of Pharmacy, Department of Chemistry in Pharmacy, Zmaja od Bosne 8, Sarajevo, Bosnia and Herzegovina
Anesa Jerković-Mujkić ; University of Sarajevo, Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, Sarajevo, Bosnia and Herzegovina

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Abstract

Sloe (Prunus spinosa L.) extracts are a good source of natural bioactive compounds, including phytosterols. Phytosterols are known to be applied in the treatment of various prostate diseases. The in vitro antiproliferative activity of sloe ethanolic extracts (flower, leaf, and fruit), collected from three areas in Bosnia and Herzegovina, were investigated against human prostate cancer cell lines PC-3 and DU145 using MTT assay. β-sitosterol, campesterol and stigmasterol were quantified by HPLC-PDA analysis using Symmetry C18 chromatographic column. The results of the analysis proved the presence of phytosterols, mostly β-sitosterol in all extracts. All extracts possess antiproliferative activity. The highest activity against PC-3 and DU145 was gathered from leaf extracts obtained by different extraction methods (microwave-assisted extraction and ultrasound-assisted extraction). To the best of our knowledge, no other studies have presented results on antiproliferative activity of ethanol sloe extracts. Based on these results, further investigation should be recommended on other cancer cell lines as well.

Keywords

HPLC; phytosterols; sloe; cytotoxicity; PC-3; DU145

Hrčak ID:

301960

URI

https://hrcak.srce.hr/301960

Publication date:

17.5.2023.

Article data in other languages: croatian

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