Original scientific paper
COMPARISON BETWEEN ELISA AND TLC/HPLC METHODS FOR DETERMINATION OF ZEARALENON AND OCHRATOXIN A IN FOOD AND FEED
Maja Šegvić Klarić
; Farmaceutsko-biokemijski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Stjepan Pepeljnjak
; Farmaceutsko-biokemijski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Zdenka Cvetnić
; Farmaceutsko-biokemijski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Ivan Kosalec
; Farmaceutsko-biokemijski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Abstract
Fusarium and Penicillium are the most common mould species that contaminate cereals in the mild climatic zone. Therefore, zearalenone (ZEA) and ochratoxin A (OTA) are frequently detected in these substrates in different concentrations depending on storage and microclimatic conditions. The purpose of this study was to determine the frequency and concentrations of ZEA and OTA in cereal and feed samples randomly collected in individual households of endemic nephropathy region (EN) in Croatia. Direct competitive ELISA test kit was used for ZEA and OTA determination according to manufacturer instructions. Results of ELISA method as well as limits of detection (LOD) and quantification were compared with high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) methods. Correlation between results was also analysed. ZEA was detected in 91.9% of samples ranging between 12.5 and 1168µg/kg (mean 318.3 µg/kg), while OTA was recovered from 16,2% of samples in concentrations ranging from 2.5 µg/kg to 31.7 µg/kg
(mean 9.8 µg/kg). ZEA and OTA simultaneously occurred in 13.5 % of samples. Detected values of mycotoxins were under the permissible levels according to Croatian law (Narodne novine No. 118/2007 and European Commission (EC/576/2006 and EC/1881/2006). The lowest LOD was obtained by ELISA followed by HPLC. Mycotoxin concentrations detected by ELISA significantly correlate with results obtained by HPLC. ELISA provide an attractive and promising method for OTA and ZEA detection in food due to high specificity, sensitivity, simplicity (analysis of high number of samples) and it is ecologically more acceptable than chromatographic methods. Advantage of HPLC method is multiple mycotoxin detection and quantification in one sample.
Keywords
ELISA i TLC/HPLC methods; zearalenon; ochratoxin
Hrčak ID:
32670
URI
Publication date:
22.12.2008.
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