Original scientific paper

Clenbuterol Residues in Plasma and Urine Samples of Food-Producing Pigs During and After Subchronic Exposure to a Growth-Promoting Dose

Jelka Pleadin orcid.org/0000-0002-0768-0462 ; Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR-10 000 Zagreb, Croatia
Tihomira Gojmerac ; Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR-10 000 Zagreb, Croatia
Igor Bratoš ; Pliva Research Institute, Prilaz Baruna Filipovića 29, HR-10 000 Zagreb, Croatia
Zoran Lipej ; Laboratory for Pathology, Croatian Veterinary Institute, Savska 143, HR-10 000 Zagreb, Croatia
Dinko Novosel ; Laboratory for Pathology, Croatian Veterinary Institute, Savska 143, HR-10 000 Zagreb, Croatia
Ana Vulić ; Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR-10 000 Zagreb, Croatia

Abstract

The aim of the study is to evaluate the suitability of plasma and urine as matrices for clenbuterol residue determination during and after its subchronic administration at a growth-promoting dose to male pigs, using previously validated enzyme-linked immunosorbent assay (ELISA) as a screening method and liquid chromatography tandem mass spectrometry (LC-MS/MS) as a confirmation method. A high correlation coefficient between these analytical methods was obtained for both urine (R=0.9800) and plasma (R=0.9970) concentrations. Study results show the plasma and urine concentration to vary greatly during oral treatment with clenbuterol for 28 days. The peak urine concentration ((88.54±50.54) ng/mL) recorded on day 21 was 40-fold peak plasma concentration ((2.25±1.54) ng/mL). After withdrawal period, the peak urine clenbuterol concentration ((42.93±10.52) ng/mL) recorded on day 0 was 24-fold plasma concentration ((1.79±0.97) ng/mL). The maximum allowed concentration of 0.5 ng/g in the liver as a regulated matrix for control of clenbuterol abuse was achieved in plasma on day 3 ((0.52±0.26) ng/mL) and in urine on day 7 of treatment withdrawal ((0.45±0.11) ng/mL). Study results indicate that urine and plasma may be suitable matrices for the control of clenbuterol abuse during fattening of food-producing pigs but have a limited value because of the rapidly decreasing concentration upon treatment withdrawal, in plasma in particular.

Keywords

clenbuterol residues; growth promoting dose; subchronic exposure; pig; urine; plasma

Hrčak ID:

33055

URI

https://hrcak.srce.hr/33055

Publication date:

26.3.2009.

Article data in other languages: croatian

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