Original scientific paper
A Study of Antifungal and Antiafl atoxigenic Action of Newly Synthesized Analogues of Dehydroacetic Acid
Duarković Lejla
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Mihaela Blažinkov
; Faculty of Agriculture, Zagreb, Croatia
Sanja Sikora
; Faculty of Agriculture, Zagreb, Croatia
Frane Delaš
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Andrea Skelin
; Faculty of Agriculture, Zagreb, Croatia
Alma Tudić
; Croatian Waters, Zagreb, Croatia
Mirna Mrkonjić-Fuka
; Faculty of Agriculture, Zagreb
Marijan Bošnjak
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Abstract
Mycelial growth and afl atoxin B1 accumulation by toxigenous mould Aspergillus flavus were inhibited by dehydroacetic acid and its newly
synthesized analogues, 4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2H-yrane-2-one (DHT) and 5-Bromo-4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2Hpyrane-2-one (BrDHT) during the growth of investigated mould on solid substrate (soy-beans). Experiments were carried out in a stationary culture at 20 0C and 30 0C, respectively, during 21 days. Mycelial growth (biomass dry weight) was determined by measuring the chitin content and concentration of afl atoxin B1 (AFB1) was quantitated by HPLC. Concentrations of DHA and
DHT of 0.1 and 0.2 μg x g-1 of substrate were stimulated, but concentrations higher than 0.5 μg x g-1 of these compounds, mould growth and AFB1 accumulation partially inhibited. In experiments with 0.2 μg x g-1 of BrDHT mould growth and AFB1 accumulation were inhibited completely depending on the time and temperature of cultivation.
Keywords
toxigenous moulds; aflatoxin B1; dehydroacetic acid; chitin; HPLC
Hrčak ID:
63989
URI
Publication date:
31.12.2010.
Visits: 1.516 *