Veterinarski arhiv, Vol. 93 No. 6, 2023.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.1907
The protective effects of Nigella sativa, thymoquinone and bentonite in experimental aflatoxicosis model in broilers
Burak Dik
orcid.org/0000-0003-2738-6911
; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey
*
Mehmet Burak Ates
orcid.org/0000-0003-1297-426X
; Department of Pathology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey
Halis Oguz
orcid.org/0000-0002-9236-0630
; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey
Mustafa Ortatatli
; Department of Pathology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey
* Dopisni autor.
Sažetak
The aim of this study was to determine the preventive efficacy of black seed (Nigella sativa L.), thymoquinone and Na bentonite on biomarkers of tissue degeneration in the liver and other organs induced by aflatoxin (AF) in broilers. One hundred broiler chicks were divided into 10 equal groups and fed for 28 days. The animals received feed with 2 mg/kg total aflatoxin, Nigella sativa (NS; 5%), thymoquinone (TMQ; 300 mg/kg) and Na bentonite (BNT; 10 g/kg) both individually and in combination. At the end of the experiment, blood and liver tissue samples were collected. AF treatment significantly increased serum and liver 8-hydroxy-2’-deoxyguanosine(8-OHdG), serum transforming growth factor β (TGF-β), and liver total antioxidant capacity (TAC) levels, whereas it significantly decreased liver TGF-β and serum TAC levels compared to the control. However, there were no changes in the serum and liver hepatocyte growth factor (HGF) levels from AF. Both individual and combined addition of NS, BNT and TMQ to AF-contaminated feed significantly improved liver 8-OHdG, liver TAC and serum TGF-β levels. Serum 8-OHdG levels were ameliorated only in the AF+BNT+TMQ group. In addition, the AF+BNT+NS and AF+BNT+TMQ groups showed significant improvement in liver 8-OHdG, liver TGF-β and liver TAC levels. We conclude that NS and TMQ supplementation to AF-contaminated feed may be beneficial against aflatoxicosis in broilers.
Ključne riječi
aflatoxin; broiler; DNA damage; liver biomarker; serum biomarker
Hrčak ID:
314175
URI
Datum izdavanja:
15.1.2024.
Posjeta: 599 *