Izvorni znanstveni članak
Antioxidant Activity of Phytic Acid in Lipid Model System
Marijana Sakač
; Institut za prehrambene tehnologije, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Jasna Čanadanović-Brunet
; Katedra za primenjene i inženjerske hemije, Tehnološki fakultet, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Aleksandra Mišan
; Institut za prehrambene tehnologije, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Vesna Tumbas
; Katedra za primenjene i inženjerske hemije, Tehnološki fakultet, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Đorđe Medić
; Institut za prehrambene tehnologije, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Sažetak
Free radicals formed during thermal (60 °C, 24 h) and catalytic (Fe2+ ions, room temperature (23±1) °C, 3 h) oxidative degradation of hydroperoxyde-enriched soybean oil (HESO) were stabilized in the presence of spin trap N-tert-butyl-α-phenylnitrone (PBN) and detected by electron spin resonance (ESR) spectrometry. In both thermal and catalytic oxidation of HESO, the same hyperfine coupling parameters (aN=14.75 G and aH β=2.80 G) confirmed the generation of PBN-OOL/-OL spin adducts. The antiradical activity (AA) of phytic acid, in the 0.076–0.30 mM concentration range, was tested by measuring its ability to inhibit the generation of PBN-OOL/-OL spin adducts during thermal and catalytic oxidation of HESO. Phytic acid did not inhibit the thermal oxidation of HESO and showed no effect in the β-carotene bleaching test (AOA). Contrary to this, phytic acid exhibited antioxidant effect on the catalytic oxidation of HESO by chelating Fe2+ ions. The mechanism of antioxidant activity was confirmed by the results of chelating activity on Fe2+ in Fe2+-ferrozine test where a dose-dependent chelating activity of phytic acid was obtained.
Ključne riječi
phytic acid; lipid oxyradicals; antioxidant activity; ESR; chelating activity
Hrčak ID:
61724
URI
Datum izdavanja:
15.12.2010.
Posjeta: 2.483 *