Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2020.61.55
The effects of daylight exposure on melatonin levels, Kiss1 expression, and melanoma formation in mice
Percin Pazarci
orcid.org/0000-0002-3708-0054
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Halil Kaplan
; Department of Pharmacology, Çukurova University Faculty of Medicine, Adana, Turkey
Davut Alptekin
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Mehmet Yilmaz
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Umit Lüleyap
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Ergin Singirik
; Department of Pharmacology, Çukurova University Faculty of Medicine, Adana, Turkey
Aykut Pelit
; Department of Biophysics, Çukurova University Faculty of Medicine, Adana, Turkey
Halil Kasap
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Arash Yegani
; Department of Pharmacology and Toxicology, Hatay Mustafa Kemal University Faculty of Veterinary Medicine, Antakya, Turkey
Sažetak
Aim To determine how daylight exposure in mice affects
melatonin protein expression in blood and Kiss1 gene expression in the hypothalamus. The second aim was to assess the relationship between skin cancer formation, daylight exposure, melatonin blood level, and kisspeptin gene
expression level.
Methods New-born mice (n=96) were assigned into the
blind group or daylight group. The blind group was raised
in the dark and the daylight group was raised under 12
hours light/12 hours dark cycle for 17 weeks. At the end of
the 11th week, melanoma cell line was inoculated to mice,
and tumor growth was observed for 6 weeks. At the end of
the experiment, melatonin level was measured from blood
serum and Kiss1 expression from the hypothalamus.
Results The blind group had significantly higher melatonin and lower Kiss1 expression levels than the daylight
group. Tumor volume was inversely proportional to melatonin levels and directly proportional to Kiss1 expression
levels. Tumor growth speed was lower in the blind than in
the daylight group.
Conclusion Melatonin and Kiss1 were shown to be
nvolved in tumor suppression. They were affected by daylight and were mutually affected by each other.
Ključne riječi
Hrčak ID:
278014
URI
Datum izdavanja:
24.12.2020.
Posjeta: 754 *