Veterinarska stanica, Vol. 54 No. 6, 2023.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.54.6.9
Cytinus hypocistis (L.) L. extract effects in an animal model of papillomavirus-induced neoplasia
Catarina Medeiros
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Ana Rita Silva
; Mountain Research Center (CIMO), Polytechnic Institute of Bragança (IPB), Campus de Santa Apolónia, Bragança, Portugal; Associate Laboratory for Sustainability and Technology in Mountains Regions (SusTEC), IPB, Campus de Santa Apolónia, Bragança, Portugal; Departament of Pharmaceutical Sciences, Facultad de Farmácia, CIETUS-IBSAL, Universidade de Salamanca, Salamanca, Spain
Tiago Ferreira
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Lillian Barros
; Mountain Research Center (CIMO), Polytechnic Institute of Bragança (IPB), Campus de Santa Apolónia, Bragança, Portugal; Associate Laboratory for Sustainability and Technology in Mountains Regions (SusTEC), IPB, Campus de Santa Apolónia, Bragança, Portugal
Maria J. Neuparth
; Research Center in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sports - University of Porto (FADEUP), Laboratory for Integrative and Translational Research in Population Health (ITR), Porto, Portugal; Toxicology Research Unit (TOXRUN), University Institute of Health Sciences, CESPU, Gandra, Portugal
Francisco Peixoto
; Chemistry Research Center (CQVR), UTAD, Vila Real, Portugal
Margarida M. S. M. Bastos
; Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Faculty of Engineering, University of Porto, Porto, Portugal; Associate Laboratory in Chemical Engineering (ALiCE), Faculty of Engineering, University of Porto, Porto, Portugal
Rui Medeiros
; Molecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Porto, Portugal; Biomedicine Research Center (CEBIMED), Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal; Research Department, LPCC-Portuguese League Against Cancer (NRNorte), Estr. da Circunvalação, Porto, Portugal; Faculty of Medicine, University of Porto, Porto, Portugal; Institute of Biomedical Sciences Abel Salazar (ICBAS), Porto, Portugal
Rui M. Gil da Costa
orcid.org/0000-0002-2151-2449
; Molecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Porto, Portugal; Biomedicine Research Center (CEBIMED), Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal; Research Department, LPCC-Portuguese League Against Cancer (NRNorte), Estr. da Circunvalação, Porto, Portugal; Faculty of Medicine, University of Porto, Porto, Portugal; Institute of Biomedical Sciences Abel Salazar (ICBAS), Porto, Portugal; Post-graduate Programme in Adult Health (PPGSAD), Morphology Department and University Hospital (HUUFMA), Federal University of Maranhão (UFMA), São Luís, Brazil
Maria João Pires
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Department of Veterinary Sciences, UTAD, Vila Real, Portugal
Ana I. Faustino-Rocha
orcid.org/0000-0001-5572-6317
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Department of Zootechnics, School of Sciences and Technology, University of Évora, Évora, Portugal; Comprehensive Health Research Center, Évora, Portugal; Adelina GAMA, Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Adelina Gama
; Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Paula Alexandra Oliveira
orcid.org/0000-0001-9519-4044
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Department of Veterinary Sciences, UTAD, Vila Real, Portugal
Sažetak
Infections with certain types of papillomavirus, such as the human papillomavirus 16 (HPV16), are associated with the development of preneoplastic lesions and cancers of the anogenital, and head and neck regions. Cytinus hypocistis (L.) L. extracts are composed of substances presenting antiproliferative, antioxidant, anti-inflammatory and antibacterial properties, which might be promising as new therapeutic compounds. This study analysed the influence of topical application of an extract obtained from C. hypocistis (CH) on K14-HPV16 and FVB/n mice to evaluate its therapeutic and toxicological properties. To achieve the study goals, 30 female mice, 33–37 weeks old, were divided into six groups (n=5/group): I (HPV+CH3.1); II (HPV+CH6.2); III (HPV+CH12.4); IV (FVB/n+CH12.4); V (HPV+control) and VI (FVB/n+-control). CH was applied topically to both ears for 28 days. After this period, all animals were sacrificed for samples collection. Skin lesions were classified histologically. Toxicological parameters included haematological and biochemical blood markers, and hepatic oxidative stress analysis. Transgenic animals showed a decrease in mean body weight regardless of the concentration of extract applied. The extract had no influence on physiological parameters, organ weight, or biochemical and oxidative stress parameters. Histology demonstrated the presence of proliferative epithelial lesions in the skin and oral mucosa of K14-HPV16 mice, with no association with the application of this extract. Overall, the application of CH extract had no influence on the skin lesions and was well tolerated by the animals in these concentrations.
Ključne riječi
animal model; mouse; natural compound; toxicology
Hrčak ID:
296405
URI
Datum izdavanja:
19.5.2023.
Posjeta: 908 *