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Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.

Momein H. El-Katatny ; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Ahmed M. Hetta ; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Gehan M. Shaban ; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Hesham M. El-Komy ; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt

Puni tekst: engleski, pdf (106 KB) str. 219-225 preuzimanja: 659* citiraj
APA 6th Edition
El-Katatny, M.H., Hetta, A.M., Shaban, G.M. i El-Komy, H.M. (2003). Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.. Food Technology and Biotechnology, 41 (3), 219-225. Preuzeto s https://hrcak.srce.hr/118617
MLA 8th Edition
El-Katatny, Momein H., et al. "Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.." Food Technology and Biotechnology, vol. 41, br. 3, 2003, str. 219-225. https://hrcak.srce.hr/118617. Citirano 19.10.2021.
Chicago 17th Edition
El-Katatny, Momein H., Ahmed M. Hetta, Gehan M. Shaban i Hesham M. El-Komy. "Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.." Food Technology and Biotechnology 41, br. 3 (2003): 219-225. https://hrcak.srce.hr/118617
Harvard
El-Katatny, M.H., et al. (2003). 'Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.', Food Technology and Biotechnology, 41(3), str. 219-225. Preuzeto s: https://hrcak.srce.hr/118617 (Datum pristupa: 19.10.2021.)
Vancouver
El-Katatny MH, Hetta AM, Shaban GM, El-Komy HM. Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.. Food Technology and Biotechnology [Internet]. 2003 [pristupljeno 19.10.2021.];41(3):219-225. Dostupno na: https://hrcak.srce.hr/118617
IEEE
M.H. El-Katatny, A.M. Hetta, G.M. Shaban i H.M. El-Komy, "Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.", Food Technology and Biotechnology, vol.41, br. 3, str. 219-225, 2003. [Online]. Dostupno na: https://hrcak.srce.hr/118617. [Citirano: 19.10.2021.]

Sažetak
Conidia of three Trichoderma isolates were formulated to make alginate pellets with or without 0.5 % chitin or dried fungal mycelium of Fusarium oxysporum as carbon source. The formulations were compared for their ability of in vitro chitinase and β-1,3-glucanase
production with free fungal spore suspensions. Conidia entrapped in alginate with or without adjuvant showed high production of enzymes (especially for chitinase) even when repeated 4 times. The addition of chitin or dried fungal mycelium as adjuvant enhanced the enzyme production up to 5 and 2-fold for chitinase and β-1,3-glucanase, respectively. Alginate concentration and surface area of the beads affected the enzyme production. The optimum initial pH, incubation time and temperature were pH=6, 12 days and 40 °C for chitinase, and pH=7, 10 days and 35 °C for β-1,3-glucanase production. The improvement of cell wall degrading enzyme production by alginate encapsulated Trichoderma could explain the in vivo inhibitory effect of such formulations on the target phytopathogenic fungi.

Ključne riječi
Trichoderma spp.; biocontrol; chitinase; glucanase, immobilization; Ca-alginate

Hrčak ID: 118617

URI
https://hrcak.srce.hr/118617

Posjeta: 988 *