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Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation

Zlata Štefanac ; Hrvatska
Mladen Krajačić ; Hrvatska

Puni tekst: engleski, pdf (216 KB) str. 13-17 preuzimanja: 128* citiraj
APA 6th Edition
Štefanac, Z. i Krajačić, M. (1991). Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation. Acta Botanica Croatica, 50 (1), 13-17. Preuzeto s https://hrcak.srce.hr/159701
MLA 8th Edition
Štefanac, Zlata i Mladen Krajačić. "Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation." Acta Botanica Croatica, vol. 50, br. 1, 1991, str. 13-17. https://hrcak.srce.hr/159701. Citirano 20.10.2021.
Chicago 17th Edition
Štefanac, Zlata i Mladen Krajačić. "Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation." Acta Botanica Croatica 50, br. 1 (1991): 13-17. https://hrcak.srce.hr/159701
Harvard
Štefanac, Z., i Krajačić, M. (1991). 'Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation', Acta Botanica Croatica, 50(1), str. 13-17. Preuzeto s: https://hrcak.srce.hr/159701 (Datum pristupa: 20.10.2021.)
Vancouver
Štefanac Z, Krajačić M. Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation. Acta Botanica Croatica [Internet]. 1991 [pristupljeno 20.10.2021.];50(1):13-17. Dostupno na: https://hrcak.srce.hr/159701
IEEE
Z. Štefanac i M. Krajačić, "Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation", Acta Botanica Croatica, vol.50, br. 1, str. 13-17, 1991. [Online]. Dostupno na: https://hrcak.srce.hr/159701. [Citirano: 20.10.2021.]

Sažetak
We describe the use of glycerol to reduce aggregation of tobacco streak ilarvirus (TSV) particles caused by formaldehyde (FA) fixation. After treatment of partially purified TSV preparations with 1% FA in the presence of 10% glycerol, double radial immunodiffusion tests revealed the presence of two to four times more reacting virus than in preparations treated with 1% FA only. Consequently, the method provides higher quality preparations intended for different immunochemical purposes and biophysical and biochemical analyses. We expect it to be useful in fixation of viruses which tend to aggregate in the presence of FA.

Hrčak ID: 159701

URI
https://hrcak.srce.hr/159701

[hrvatski]

Posjeta: 288 *