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Food Technology and Biotechnology, Vol.56 No.2 Lipanj 2018.

Izvorni znanstveni članak
https://doi.org/10.17113/ftb.56.02.18.5349

Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)

Sumedha Arora ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Prashant Mohanpuria ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Gurupkar Singh Sidhu ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Inderjit Singh Yadav ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Vandna Kumari ; Botany Department, Punjabi University, IN-147002 Patiala, India

Puni tekst: engleski, pdf (6 MB) str. 228-237 preuzimanja: 0* citiraj
APA 6th Edition
Arora, S., Mohanpuria, P., Singh Sidhu, G., Singh Yadav, I. i Kumari, V. (2018). Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco). Food Technology and Biotechnology, 56 (2), 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
MLA 8th Edition
Arora, Sumedha, et al. "Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)." Food Technology and Biotechnology, vol. 56, br. 2, 2018, str. 228-237. https://doi.org/10.17113/ftb.56.02.18.5349. Citirano 22.07.2018.
Chicago 17th Edition
Arora, Sumedha, Prashant Mohanpuria, Gurupkar Singh Sidhu, Inderjit Singh Yadav i Vandna Kumari. "Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)." Food Technology and Biotechnology 56, br. 2 (2018): 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
Harvard
Arora, S., et al. (2018). 'Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)', Food Technology and Biotechnology, 56(2), str. 228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
Vancouver
Arora S, Mohanpuria P, Singh Sidhu G, Singh Yadav I, Kumari V. Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco). Food Technology and Biotechnology [Internet]. 29.06.2018. [pristupljeno 22.07.2018.];56(2):228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
IEEE
S. Arora, P. Mohanpuria, G. Singh Sidhu, I. Singh Yadav i V. Kumari, "Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)", Food Technology and Biotechnology, vol.56, br. 2, str. 228-237, Srpanj 2018. [Online]. doi: https://doi.org/10.17113/ftb.56.02.18.5349
Puni tekst: hrvatski, pdf (6 MB) str. 228-237 preuzimanja: 0* citiraj
APA 6th Edition
Arora, S., Mohanpuria, P., Singh Sidhu, G., Singh Yadav, I. i Kumari, V. (2018). Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco). Food Technology and Biotechnology, 56 (2), 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
MLA 8th Edition
Arora, Sumedha, et al. "Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)." Food Technology and Biotechnology, vol. 56, br. 2, 2018, str. 228-237. https://doi.org/10.17113/ftb.56.02.18.5349. Citirano 22.07.2018.
Chicago 17th Edition
Arora, Sumedha, Prashant Mohanpuria, Gurupkar Singh Sidhu, Inderjit Singh Yadav i Vandna Kumari. "Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)." Food Technology and Biotechnology 56, br. 2 (2018): 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
Harvard
Arora, S., et al. (2018). 'Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)', Food Technology and Biotechnology, 56(2), str. 228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
Vancouver
Arora S, Mohanpuria P, Singh Sidhu G, Singh Yadav I, Kumari V. Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco). Food Technology and Biotechnology [Internet]. 29.06.2018. [pristupljeno 22.07.2018.];56(2):228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
IEEE
S. Arora, P. Mohanpuria, G. Singh Sidhu, I. Singh Yadav i V. Kumari, "Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)", Food Technology and Biotechnology, vol.56, br. 2, str. 228-237, Srpanj 2018. [Online]. doi: https://doi.org/10.17113/ftb.56.02.18.5349

Sažetak
Kinnow mandarin (Citrus reticulata Blanco) is a popular citrus crop of northwestern India and it occupies maximum fruit area in Punjab. However, citrus juice processing industry is still suffering from delayed bitterness problem caused mainly by limonoid aglycones such as limonin. In order to study citrus limonoid metabolism, limonoid glucosyltransferase (LGT) gene, which encodes a natural debittering enzyme, was isolated from the fruit tissues of Kinnow mandarin. After confirmation and characterization, its full-length gene sequence (1533 bp) was submitted to National Centre for Biotechnology Information. Citrus reticulata limonoid glucosyltransferase (CrLGT) occupies a position on an independent branch in the largest subgroup and is phylogenetically different from those in other mandarin species like C. unshiu, showing its uniqueness in several features. The transcript expression of CrLGT, evaluated in different tissues such as young leaf, flavedo, albedo, sac covering and seed of Kinnow mandarin during early (90 days after flowering (DAF)), mid (150-210 DAF) and late (240 DAF) fruit developmental stages using semi-quantitative method, showed the highest expression in flavedo. Thus, it was concluded that the isolated LGT gene has an effect on limonoid metabolic engineering in citrus. Overexpression of this gene can reduce the delayed bitterness problem in citrus juice and enhance the accumulation of specific glucosides that have anticancer effects.

Ključne riječi
Kinnow mandarin; delayed bitterness; limonoid glucosyltransferase; anticancer properties; semi-quantitative PCR

Hrčak ID: 203490

URI
https://hrcak.srce.hr/203490

[hrvatski]

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