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Food Technology and Biotechnology, Vol.56 No.2 Lipanj 2018.

Kratko priopćenje
https://doi.org/10.17113/ftb.56.02.18.5393

Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments

Ranko Gacesa ; Department of Chemistry, King’s College London, Franklin-Wilkins Building, Stamford Street, London SE1 9NH, UK
Damir Baranasic ; Department of Genetics, University of Kaiserslautern, Postfach 3049, DE-67653 Kaiserslautern, Germany
Antonio Starcevic ; Centre of Research Excellence for Marine Bioprospecting - BioProCro, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Janko Diminic ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Marino Korlević ; Centre for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, HR- 52210 Rovinj, Croatia
Mirjana Najdek ; Centre for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, HR- 52210 Rovinj, Croatia
Maria Blažina ; Centre for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, HR- 52210 Rovinj, Croatia
Davor Oršolić ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Domagoj Kolesarić ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Paul F. Long ; Department of Chemistry, King’s College London, Franklin-Wilkins Building, Stamford Street, London SE1 9NH, UK
John Cullum ; Department of Genetics, University of Kaiserslautern, Postfach 3049, DE-67653 Kaiserslautern, Germany
Daslav Hranueli ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Sandi Orlic ; Center of Excellence for Science and Technology Integrating Mediterranean Region, Microbial Ecology, HR-10000 Zagreb, Croatia
Jurica Zucko ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (972 KB) str. 270-277 preuzimanja: 0* citiraj
APA 6th Edition
Gacesa, R., Baranasic, D., Starcevic, A., Diminic, J., Korlević, M., Najdek, M., ... Zucko, J. (2018). Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments. Food Technology and Biotechnology, 56 (2), 270-277. https://doi.org/10.17113/ftb.56.02.18.5393
MLA 8th Edition
Gacesa, Ranko, et al. "Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments." Food Technology and Biotechnology, vol. 56, br. 2, 2018, str. 270-277. https://doi.org/10.17113/ftb.56.02.18.5393. Citirano 21.07.2018.
Chicago 17th Edition
Gacesa, Ranko, Damir Baranasic, Antonio Starcevic, Janko Diminic, Marino Korlević, Mirjana Najdek, Maria Blažina, et al. "Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments." Food Technology and Biotechnology 56, br. 2 (2018): 270-277. https://doi.org/10.17113/ftb.56.02.18.5393
Harvard
Gacesa, R., et al. (2018). 'Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments', Food Technology and Biotechnology, 56(2), str. 270-277. doi: https://doi.org/10.17113/ftb.56.02.18.5393
Vancouver
Gacesa R, Baranasic D, Starcevic A, Diminic J, Korlević M, Najdek M i sur. Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments. Food Technology and Biotechnology [Internet]. 29.06.2018. [pristupljeno 21.07.2018.];56(2):270-277. doi: https://doi.org/10.17113/ftb.56.02.18.5393
IEEE
R. Gacesa, et al., "Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments", Food Technology and Biotechnology, vol.56, br. 2, str. 270-277, Srpanj 2018. [Online]. doi: https://doi.org/10.17113/ftb.56.02.18.5393
Puni tekst: hrvatski, pdf (972 KB) str. 270-277 preuzimanja: 0* citiraj
APA 6th Edition
Gacesa, R., Baranasic, D., Starcevic, A., Diminic, J., Korlević, M., Najdek, M., ... Zucko, J. (2018). Biološka raznolikost gena što sadržavaju genetičku uputu za enzime koji razgrađuju ugljikovodike iz uzoraka metagenoma izoliranih iz sedimenata sjevernog Jadranskog mora. Food Technology and Biotechnology, 56 (2), 270-277. https://doi.org/10.17113/ftb.56.02.18.5393
MLA 8th Edition
Gacesa, Ranko, et al. "Biološka raznolikost gena što sadržavaju genetičku uputu za enzime koji razgrađuju ugljikovodike iz uzoraka metagenoma izoliranih iz sedimenata sjevernog Jadranskog mora." Food Technology and Biotechnology, vol. 56, br. 2, 2018, str. 270-277. https://doi.org/10.17113/ftb.56.02.18.5393. Citirano 21.07.2018.
Chicago 17th Edition
Gacesa, Ranko, Damir Baranasic, Antonio Starcevic, Janko Diminic, Marino Korlević, Mirjana Najdek, Maria Blažina, et al. "Biološka raznolikost gena što sadržavaju genetičku uputu za enzime koji razgrađuju ugljikovodike iz uzoraka metagenoma izoliranih iz sedimenata sjevernog Jadranskog mora." Food Technology and Biotechnology 56, br. 2 (2018): 270-277. https://doi.org/10.17113/ftb.56.02.18.5393
Harvard
Gacesa, R., et al. (2018). 'Biološka raznolikost gena što sadržavaju genetičku uputu za enzime koji razgrađuju ugljikovodike iz uzoraka metagenoma izoliranih iz sedimenata sjevernog Jadranskog mora', Food Technology and Biotechnology, 56(2), str. 270-277. doi: https://doi.org/10.17113/ftb.56.02.18.5393
Vancouver
Gacesa R, Baranasic D, Starcevic A, Diminic J, Korlević M, Najdek M i sur. Biološka raznolikost gena što sadržavaju genetičku uputu za enzime koji razgrađuju ugljikovodike iz uzoraka metagenoma izoliranih iz sedimenata sjevernog Jadranskog mora. Food Technology and Biotechnology [Internet]. 29.06.2018. [pristupljeno 21.07.2018.];56(2):270-277. doi: https://doi.org/10.17113/ftb.56.02.18.5393
IEEE
R. Gacesa, et al., "Biološka raznolikost gena što sadržavaju genetičku uputu za enzime koji razgrađuju ugljikovodike iz uzoraka metagenoma izoliranih iz sedimenata sjevernog Jadranskog mora", Food Technology and Biotechnology, vol.56, br. 2, str. 270-277, Srpanj 2018. [Online]. doi: https://doi.org/10.17113/ftb.56.02.18.5393

Sažetak
Three metagenomic libraries were constructed using surface sediment samples from the northern Adriatic Sea. Two of the samples were taken from a highly polluted and an unpolluted site respectively. The third sample from a polluted site had been enriched using crude oil. The results of the metagenome analyses were incorporated in the REDPET relational database (http://redpet.bioinfo.pbf.hr/REDPET), which was generated using the previously developed MEGGASENSE platform. The database includes taxonomic data to allow the assessment of the biodiversity of metagenomic libraries and a general functional analysis of genes using hidden Markov model (HMM) profiles based on the KEGG database. A set of 22 specialised HMM profiles was developed to detect putative genes for hydrocarbon-degrading enzymes. Use of these profiles showed that the metagenomic library generated after selection on crude oil had enriched genes for aerobic n-alkane degradation. The use of this system for bioprospecting was exemplified using potential alkB and almA genes from this library.

Ključne riječi
oil pollution; n-alkane degradation; database

Projekti
HRZZ / IS / IS-09/5 - Rizici zagađenja Jadranskog mora naftom

Hrčak ID: 203496

URI
https://hrcak.srce.hr/203496

[hrvatski]

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