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Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli

Luka Bočkor ; ICGEB, AREA Science Park, Padriciano 99, IT-34149 Trieste, Italy
Srećko Jelenić† ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Horvatovac 102a, HR-10000 Zagreb, Croatia
Nenad Malenica ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Horvatovac 102a, HR-10000 Zagreb, Croatia
Jelena Mlinarec ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Horvatovac 102a, HR-10000 Zagreb, Croatia
Višnja Besendorfer ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ivana Ivančić-Baće ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Horvatovac 102a, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (193 KB) str. 441-445 preuzimanja: 247* citiraj
APA 6th Edition
Bočkor, L., Jelenić†, S., Malenica, N., Mlinarec, J., Besendorfer, V. i Ivančić-Baće, I. (2013). Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli. Food Technology and Biotechnology, 51 (4), 441-445. Preuzeto s https://hrcak.srce.hr/114446
MLA 8th Edition
Bočkor, Luka, et al. "Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli." Food Technology and Biotechnology, vol. 51, br. 4, 2013, str. 441-445. https://hrcak.srce.hr/114446. Citirano 20.09.2019.
Chicago 17th Edition
Bočkor, Luka, Srećko Jelenić†, Nenad Malenica, Jelena Mlinarec, Višnja Besendorfer i Ivana Ivančić-Baće. "Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli." Food Technology and Biotechnology 51, br. 4 (2013): 441-445. https://hrcak.srce.hr/114446
Harvard
Bočkor, L., et al. (2013). 'Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli', Food Technology and Biotechnology, 51(4), str. 441-445. Preuzeto s: https://hrcak.srce.hr/114446 (Datum pristupa: 20.09.2019.)
Vancouver
Bočkor L, Jelenić† S, Malenica N, Mlinarec J, Besendorfer V, Ivančić-Baće I. Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli. Food Technology and Biotechnology [Internet]. 2013 [pristupljeno 20.09.2019.];51(4):441-445. Dostupno na: https://hrcak.srce.hr/114446
IEEE
L. Bočkor, S. Jelenić†, N. Malenica, J. Mlinarec, V. Besendorfer i I. Ivančić-Baće, "Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli", Food Technology and Biotechnology, vol.51, br. 4, str. 441-445, 2013. [Online]. Dostupno na: https://hrcak.srce.hr/114446. [Citirano: 20.09.2019.]

Sažetak
In this work we have constructed a plasmid to compare intraplasmid recombination efficiency in Agrobacterium tumefaciens and Escherichia coli. The plasmid contains two directly repeated copies of spectinomycin resistance gene, one lacking 5’ and the other lacking 3’ end. These two copies share a 570-bp region of homology and are separated by the ampicillin resistance gene. Homologous recombination between repeated copies of incomplete spectinomycin resistance genes results in the restoration of spectinomycin resistance. During this process, ampicillin resistance gene is either deleted or incomplete spectinomycin genes are amplified along with the ampicillin resistance gene. This experimental system enabled us to follow for the first time the generation of deletions and amplifications during intraplasmid recombination in A. tumefaciens. We show here that predominantly RecA-independent mechanism contributes to the formation of deletion and amplification products in both, A. tumefaciens and E. coli. Additionally, deletion and amplification products were detected at similar frequencies, suggesting that amplifications and deletions probably occur by a similar mechanism.

Ključne riječi
RecA; intramolecular recombination; Agrobacterium tumefaciens; Escherichia coli

Hrčak ID: 114446

URI
https://hrcak.srce.hr/114446

[hrvatski]

Posjeta: 450 *