hrcak mascot   Srce   HID

Food Technology and Biotechnology, Vol. 55 No. 1, 2017.

Izvorni znanstveni članak
https://doi.org/10.17113/ftb.55.01.17.4693

Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium

Mia Potočnjak ; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Petra Pušić ; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Jadranka Frece ; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for General Microbiology and Food Microbiology, Pierottijeva 6, HR-10000 Zagreb, Croatia
Maja Abram ; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Tamara Janković ; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Ivana Gobin   ORCID icon orcid.org/0000-0002-8956-4675 ; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia

Puni tekst: engleski, pdf (418 KB) str. 48-54 preuzimanja: 112* citiraj
APA 6th Edition
Potočnjak, M., Pušić, P., Frece, J., Abram, M., Janković, T. i Gobin, I. (2017). Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium. Food Technology and Biotechnology, 55 (1), 48-54. https://doi.org/10.17113/ftb.55.01.17.4693
MLA 8th Edition
Potočnjak, Mia, et al. "Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium." Food Technology and Biotechnology, vol. 55, br. 1, 2017, str. 48-54. https://doi.org/10.17113/ftb.55.01.17.4693. Citirano 22.04.2019.
Chicago 17th Edition
Potočnjak, Mia, Petra Pušić, Jadranka Frece, Maja Abram, Tamara Janković i Ivana Gobin. "Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium." Food Technology and Biotechnology 55, br. 1 (2017): 48-54. https://doi.org/10.17113/ftb.55.01.17.4693
Harvard
Potočnjak, M., et al. (2017). 'Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium', Food Technology and Biotechnology, 55(1), str. 48-54. doi: https://doi.org/10.17113/ftb.55.01.17.4693
Vancouver
Potočnjak M, Pušić P, Frece J, Abram M, Janković T, Gobin I. Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium. Food Technology and Biotechnology [Internet]. 2017 [pristupljeno 22.04.2019.];55(1):48-54. doi: https://doi.org/10.17113/ftb.55.01.17.4693
IEEE
M. Potočnjak, P. Pušić, J. Frece, M. Abram, T. Janković i I. Gobin, "Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium", Food Technology and Biotechnology, vol.55, br. 1, str. 48-54, 2017. [Online]. doi: https://doi.org/10.17113/ftb.55.01.17.4693

Rad u XML formatu

Sažetak
The benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from different dairy products: cow′s cheese, sheep′s cheese and whey. In order to determine the antibacterial activity of yet unexplored L. plantarum strains against Salmonella enterica serotype Typhimurium, in vitro competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their influence on the adhesion of Salmonella were tested. Results showed the potential probiotic activity of isolated strains. L. plantarum strains survived in the presence of 1 % bile salts, they possessed acidification ability, antibacterial activity and significantly attenuated the growth of S. Typhimurium in brain heart infusion broth. All tested L. plantarum strains were able to adhere to Caco-2 cells and significantly impair the adhesion of S. Typhimurium. All three L. plantarum strains exhibited signifi cant probiotic potential and anti-Salmonella activity; therefore, further testing on in vivo models should follow.

Ključne riječi
probiotic bacteria; antagonistic activity; Salmonella; mechanism of action

Hrčak ID: 178268

URI
https://hrcak.srce.hr/178268

Reference

1 

Marteau PR, de Vrese M, Cellier CJ, Schrezenmeir J. Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr. 2001;73(2) Suppl:430S–6S. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/11157353

2 

Soccol CR, Vandenberghe LPDS, Spier MR, Medeiros ABP, Yamaguishi CT, De Dea Lindner J, et al. The potential of probiotics: a review. Food Technol Biotechnol. 2010;48:413–34.

3 

Babić I, Markov K, Kovačević D, Trontel A, Slavica A, Đugum J, et al. Identification and characterization of potential autochthonous starter cultures from a Croatian ‘brand’ product ‘Slavonski kulen’. Meat Sci. 2011;88:517–24. DOI: http://dx.doi.org/10.1016/j.meatsci.2011.02.003 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/21377808

4 

Arena MP, Silvain A, Normanno G, Grieco F, Drider D, Spano G, et al. Use of Lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms. Front Microbiol. 2016;7:464. DOI: http://dx.doi.org/10.3389/fmicb.2016.00464 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/27148172

5 

Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria: Joint FAO/WHO Experts Consultation, October 2001. Geneva, Switzerland: Food and Agricultural Organization of the United Nations and World Health Organization (FAO/WHO): 2001. Available from: http://www.mesanders.com/probio_report.pdf.

6 

Kaushik JK, Kumar A, Duary RK, Mohanty AK, Grover S, Batish VK. Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. PLoS One. 2009;4:e8099. DOI: http://dx.doi.org/10.1371/journal.pone.0008099 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/19956615

7 

Herreros MA, Sandoval H, González L, Castro JM, Fresno JM, Tornadijo ME. Antimicrobial activity and antibiotic resistance of lactic acid bacteria isolated from Armada cheese (a Spanish goats’ milk cheese). Food Microbiol. 2005;22:455–9. DOI: http://dx.doi.org/10.1016/j.fm.2004.11.007

8 

Kos B, Beganović J, Jurašić L, Švad̄umović M, Leboš Pavunc A, Uroić K, et al. Coculture-inducible bacteriocin biosynthesis of different probiotic strains by dairy starter culture Lactococcus lactis. Mljekarstvo. 2011;61:273–82.

9 

Tejero-Sariñena S, Barlow J, Costabile A, Gibson GR, Rowland I. In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe. 2012;18:530–8. DOI: http://dx.doi.org/10.1016/j.anaerobe.2012.08.004 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/22959627

10 

Šušković J, Kos B, Frece J, Beganović J, Leboš Pavunc A. Probiotic Concept – probiotics as food supplements and probiotics as biotherapeutics. Croat J Food Technol Biotechnol Nutr. 2009;4:77–84. [in Croatian]

11 

Bernet MF, Brassart D, Neeser JR, Servin AL. Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogens-cell interactions. Appl Environ Microbiol. 1993;59:4121–8. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/8285709

12 

Dimitrov Z, Gotova I, Chorbadjiyska E. In vitro characterization of the adhesive factors of selected probiotics to Caco-2 epithelium cell line. Biotechnol Biotechnol Equip. 2014;28:1079–83. DOI: http://dx.doi.org/10.1080/13102818.2014.969948 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/26019594

13 

Myllyluoma E, Ahonen AM, Korpela R, Vapaatalo H, Kankuri E. Effects of multispecies probiotic combination on Helicobacter pylori infection in vitro. Clin Vaccine Immunol. 2008;15:1472–82. DOI: http://dx.doi.org/10.1128/CVI.00080-08 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/18579692

14 

Sribuathong S, Saengprakai J, Trevanich S. In vitro anti-adherent assessment of selected lactic acid bacteria isolates against Salmonella Typhimurium and Listeria monocytogenes to Caco-2 cells. J Food Saf. 2014;34:270–82. DOI: http://dx.doi.org/10.1111/jfs.12123

15 

Woo TDH, Oka K, Takahashi M, Hojo F, Osaki T, Hanawa T, et al. Inhibition of the cytotoxic effect of Clostridium difficile in vitro by Clostridium butyricum MIYAIRI 588 strain. J Med Microbiol. 2011;60:1617–25. DOI: http://dx.doi.org/10.1099/jmm.0.033423-0 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/21700738

16 

Henson S. Estimating the incidence of food-borne Salmonella and the effectiveness of alternative control measures using the Delphi method. Int J Food Microbiol. 1997;35:195–204. DOI: http://dx.doi.org/10.1016/S0168-1605(96)01235-4 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/9105928

17 

de Jong HK, Parry CM, van der Poll T, Wiersinga WJ. Host-pathogen interaction in invasive salmonellosis. PLoS Pathog. 2012;8:e1002933. DOI: http://dx.doi.org/10.1371/journal.ppat.1002933 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/23055923

18 

Ouwehand A, Vesterlund S. Health aspects of probiotics. IDrugs. 2003;6:573–80. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/12811680

19 

Baccigalupi L, Di Donato A, Parlato M, Luongo D, Carbone V, Rossi M, et al. Small surface-associated factors mediate adhesion of a food-isolated strain of Lactobacillus fermentum to Caco-2 cells. Res Microbiol. 2005;156:830–6. DOI: http://dx.doi.org/10.1016/j.resmic.2005.05.001 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/16125909

20 

Giannasca KT, Giannasca PJ, Neutra MR. Adherence of Salmonella typhimurium to Caco-2 cells: identification of a glycoconjugate receptor. Infect Immun. 1996;64:135–45. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/8557331

21 

Bishop A, House D, Perkins T, Baker S, Kingsley RA, Dougan G. Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion. Microbiology. 2008;154:1914–26. DOI: http://dx.doi.org/10.1099/mic.0.2008/016998-0 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/18599820

22 

Bernet-Camard MF, Liévin V, Brassart D, Neeser JR, Servin AL, Hudault S. The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo. Appl Environ Microbiol. 1997;63:2747–53. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/9212421

23 

Coconnier MH, Liévin V, Lorrot M, Servin AL. Antagonistic activity of Lactobacillus acidophilus LB against intracellular Salmonella enterica serovar Typhimurium infecting human enterocyte-like Caco-2/TC-7 cells. Appl Environ Microbiol. 2000;66:1152–7. DOI: http://dx.doi.org/10.1128/AEM.66.3.1152-1157.2000 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/10698785

24 

Servin AL. Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiol Rev. 2004;28:405–40. DOI: http://dx.doi.org/10.1016/j.femsre.2004.01.003 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/15374659

25 

Walencka E, Różalska S, Sadowska B, Różalska B. The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol (Praha). 2008;53:61–6. DOI: http://dx.doi.org/10.1007/s12223-008-0009-y PubMed: http://www.ncbi.nlm.nih.gov/pubmed/18481220

26 

Lehto EM, Salminen SJ. Inhibition of Salmonella typhimurium adhesion to Caco-2 cell cultures by Lactobacillus strain GG spent culture supernate: only a pH effect? FEMS Immunol Med Microbiol. 1997;18:125–32. DOI: http://dx.doi.org/10.1111/j.1574-695X.1997.tb01037.x PubMed: http://www.ncbi.nlm.nih.gov/pubmed/9223617

27 

Bilkova A, Kinova Sepova H, Bukovsky M, Bezakova L. Antibacterial potential of lactobacilli isolated from a lamb. Vet Med. 2011;56:319–24.

28 

Lim SM, Im DS. Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J Microbiol Biotechnol. 2009;19:178–86. DOI: http://dx.doi.org/10.4014/jmb.0804.269 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/19307768

29 

Travers MA, Florent I, Kohl L, Grellier P. Probiotics for the control of parasites: an overview. J Parasitol Res. 2011;2011:610769. DOI: http://dx.doi.org/10.1155/2011/610769 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/21966589

30 

Molin G. Probiotics in foods not containing milk or milk constituents, with special reference to Lactobacillus plantarum 299v. Am J Clin Nutr. 2001;73(2) Suppl:380S–5S. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/11157345

31 

Kumar A, Kumar D. Characterization of Lactobacillus isolated from dairy samples for probiotic properties. Anaerobe. 2015;33:117–23. DOI: http://dx.doi.org/10.1016/j.anaerobe.2015.03.004 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/25771244

32 

Frece J, Kos B, Beganović J, Vuković S, Šušković J. In vivo testing of functional properties of three selected probiotic strains. World J Microbiol Biotechnol. 2005;21:1401–8. DOI: http://dx.doi.org/10.1007/s11274-005-5741-8

33 

National Salmonella Surveillance Overview. Atlanta, GA, USA: US Department of Health and Human Services, Centers for Disease Control and Prevention; 2011.

34 

Mittrücker HW, Kaufmann SH. Immune response to infection with Salmonella typhimurium in mice. J Leukoc Biol. 2000;67:457–63. DOI: http://dx.doi.org/10.1186/1471-2334-7-65 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/10770276

35 

Andino A, Hanning I. Salmonella enterica: survival, colonization, and virulence differences among serovars. ScientificWorldJournal. 2015;2015:520179. DOI: http://dx.doi.org/10.1155/2015/520179 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/25664339

36 

Neal-McKinney JM, Lu X, Duong T, Larson CL, Call DR, Shah DH, et al. Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry. PLoS One. 2012;7:e43928. DOI: http://dx.doi.org/10.1371/journal.pone.0043928 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/22962594

37 

Tejero-Sariñena S, Barlow J, Costabile A, Gibson GR, Rowland I. In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe. 2012;18:530–8. DOI: http://dx.doi.org/10.1016/j.anaerobe.2012.08.004 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/22959627

38 

Millette M, Luquet FM, Lacroix M. In vitro growth control of selected pathogens by Lactobacillus acidophilus- and Lactobacillus casei-fermented milk. Lett Appl Microbiol. 2007;44:314–9. DOI: http://dx.doi.org/10.1111/j.1472-765X.2006.02060.x PubMed: http://www.ncbi.nlm.nih.gov/pubmed/17309510

39 

Kos B, Šušković J, Vuković S, Šimpraga M, Frece J, Matošić S. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol. 2003;94:981–7. DOI: http://dx.doi.org/10.1046/j.1365-2672.2003.01915.x PubMed: http://www.ncbi.nlm.nih.gov/pubmed/12752805

40 

Janković T, Frece J, Abram M, Gobin I. Aggregation ability of potential probiotic Lactobacillus plantarum strains. Int J Sanit Eng Res. 2012;6:19–24.

41 

Greene JD, Klaenhammer TR. Factors involved in adherence of lactobacilli to human Caco-2 cells. Appl Environ Microbiol. 1994;60:4487–94. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/7811085

42 

Frece J, Kos B, Svetec IK, Zgaga Z, Beganović J, Leboš A, et al. Synbiotic effect of Lactobacillus helveticus M92 and prebiotics on the intestinal microflora and immune system of mice. J Dairy Res. 2009;76:98–104. DOI: http://dx.doi.org/10.1017/S0022029908003737 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/19121230

43 

Jankowska A, Laubitz D, Antushevich H, Zabielski R, Grzesiuk E. Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells. J Biomed Biotechnol. 2008;2008:357964. DOI: http://dx.doi.org/10.1155/2008/357964 PubMed: http://www.ncbi.nlm.nih.gov/pubmed/18401442

[hrvatski]

Posjeta: 364 *