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https://doi.org/10.17508/CJFST.2018.10.1.11
Time-course experiment of Fusarium infestation of wheat genotypes with the emphasis on the physiological response
TIHANA MARČEK
orcid.org/0000-0002-8617-5009
; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Department of Food and Nutrition Research, F. Kuhača 20, 31000 Osijek, Croatia
MARIJA VILJEVAC VULETIĆ
orcid.org/0000-0001-8792-641X
; Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
IVAN BAKULA
; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Department of Food and Nutrition Research, F. Kuhača 20, 31000 Osijek, Croatia
SARA ALIVOJVODIĆ
; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Department of Food and Nutrition Research, F. Kuhača 20, 31000 Osijek, Croatia
VALENTINA ŠPANIĆ
orcid.org/0000-0002-7684-5419
; Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
Sažetak
Wheat (Triticum aestivum L.) is one of the most significant food cereal in the
world. Under natural conditions, biotic and abiotic stress factors can seriously
endanger the plant growth and development. Fusarium head blight (FHB),
mainly caused by F. graminearum and F. culmorum, is a disease that has
negative effects on economy, namely on the yield and the quality of the grain.
In this research, the activities of guaiacol peroxidase (POD), catalase (CAT),
ascorbate peroxidase (APX) and polyphenol oxidase (PPO), as well as H2O2
concentration and malonedialdehyde (MDA) content, were determined in
three wheat genotypes of various resistance to FHB (“Super Žitarka”-
sensitive; “Lucija”- moderately resistant, and “Apache”- resistant) after the 2nd
and the 4th day of infestation. The infected plants of “Lucija” showed higher
POD activity compared to control after the 4th day, and increased POD activity
compared to the 2nd day of infection. On the 2nd day, “Apache” responded to
pathogen by the enhanced APX activity, and higher MDA level, compared to
the 4th day of treatment when it started to decrease. Moreover, the treated
plants of the same genotype showed the increased MDA level on the 2nd day,
unlike the untreated plants. Contrarily, H2O2 concentration in “Apache”
showed the tendency to increase with the time of Fusarium-exposure. In
comparison to the 2nd day of treatment, PPO activity decreased in “Super
Žitarka” on the 4th day. In all genotypes at both sampling points, CAT activity
did not change significantly under the pathogen attack. Overproduction of
H2O2 accompanied with the increased APX activity 2 days after infection may
lead to the conclusion that FHB tolerance of “Apache” is ensured by the earlier
activation of the defence mechanisms.
Ključne riječi
wheat; Fusarium spp.; MDA; H2O2; antioxidative enzymes
Hrčak ID:
200549
URI
Datum izdavanja:
25.5.2018.
Posjeta: 1.951 *