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https://doi.org/10.32582/aa.59.2.1

Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination

Iris Batel ; Ruđer Bošković Institute, Center for Marine Research, G. Paliaga 5, 52 210 Rovinj, Croatia
Maja Fafanđel ; Ruđer Bošković Institute, Center for Marine Research, G. Paliaga 5, 52 210 Rovinj, Croatia
Mirta Smodlaka Tanković ; Ruđer Bošković Institute, Center for Marine Research, G. Paliaga 5, 52 210 Rovinj, Croatia
Ivan Ivetac ; Ruđer Bošković Institute, Center for Marine Research, G. Paliaga 5, 52 210 Rovinj, Croatia
Nevenka Bihari ; Ruđer Bošković Institute, Center for Marine Research, G. Paliaga 5, 52 210 Rovinj, Croatia

Puni tekst: engleski, pdf (1 MB) str. 161-171 preuzimanja: 93* citiraj
APA 6th Edition
Batel, I., Fafanđel, M., Smodlaka Tanković, M., Ivetac, I. i Bihari, N. (2018). Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination. Acta Adriatica, 59 (2), 161-171. https://doi.org/10.32582/aa.59.2.1
MLA 8th Edition
Batel, Iris, et al. "Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination." Acta Adriatica, vol. 59, br. 2, 2018, str. 161-171. https://doi.org/10.32582/aa.59.2.1. Citirano 18.08.2019.
Chicago 17th Edition
Batel, Iris, Maja Fafanđel, Mirta Smodlaka Tanković, Ivan Ivetac i Nevenka Bihari. "Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination." Acta Adriatica 59, br. 2 (2018): 161-171. https://doi.org/10.32582/aa.59.2.1
Harvard
Batel, I., et al. (2018). 'Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination', Acta Adriatica, 59(2), str. 161-171. https://doi.org/10.32582/aa.59.2.1
Vancouver
Batel I, Fafanđel M, Smodlaka Tanković M, Ivetac I, Bihari N. Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination. Acta Adriatica [Internet]. 2018 [pristupljeno 18.08.2019.];59(2):161-171. https://doi.org/10.32582/aa.59.2.1
IEEE
I. Batel, M. Fafanđel, M. Smodlaka Tanković, I. Ivetac i N. Bihari, "Cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes caused by environmental contamination", Acta Adriatica, vol.59, br. 2, str. 161-171, 2018. [Online]. https://doi.org/10.32582/aa.59.2.1

Sažetak
Environmental contamination includes a mixture of organic substances that can have detrimental effects on marine organisms and should be evaluated in the quality and risk assessment of investigated marine areas. Marine areas selected for this study are a protected area, a mariculture area, a shipyard and an industrial area. Based on the toxicity of the organic seawater extracts these areas were classified as an undisturbed reference area (S1), an area with the low anthropogenic impact (S2), a potentially endangered area (S3) and an area with high anthropogenic impact (S4) respectively. The organic mixtures present in seawater samples collected at the above-defined areas were tested for the induction of DNA damage and cell cycle alterations in the mussel Mytilus galloprovincialis hemocytes. Flow cytometric analyses were performed to detect changes in hemocytes DNA content distribution throughout the cell cycle. Organic seawater extracts from sampling sites S2, S3 and S4 induced an increase in the coefficient of variation of the G0/G1 peak and an increase in the number of cells in the G2/M phase reflecting the extent of DNA damage and \(G_2/M\) arrest, respectively. The \(G_2/M\) arrest in mussel hemocytes was concentration-dependent upon injection with organic seawater extracts from the S3 site and time dependant for S2, S3 and S4 sampling sites. The time dependence of the induction of the G2/M arrest showed a characteristic pattern for each site due to the different quantitative and qualitative composition of the organic seawater extracts. The \(G_2/M\) arrest was reversible 24 or 72 hours after treatment with organic seawater extracts from S2 or S3, and S4 sites, respectively. This reversibility was time- and site-specific indicating that such DNA damage is repairable to a certain degree according to the organic seawater extract composition. Thus, the hemocytes cell cycle alterations in the mussel Mytilus galloprovincialis caused by organic seawater extracts reliably reflect the extent of organic contamination effects for selected marine areas.

Ključne riječi
organic contamination; flow cytometry; DNA damage, \(G_2/M\) arrest, hemocytes; Mytilus galloprovincialis

Projekti
MZOS / 0982705-2725 - Ecotoxic effects of contamination on marine organisms

Hrčak ID: 214910

URI
https://hrcak.srce.hr/214910

[hrvatski]

Posjeta: 221 *