Kratko priopćenje
Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100
Olivera Stajković
; Laboratory for Microbiology, Institute of Soil Science, Teodora Drajzera 7, 11000 Belgrade, Serbia
Tanja Berić-Bjedov
; Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, Serbia
Dragana Mitić-Ćulafić
; Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, Serbia
Slaviša Stanković
; Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, Serbia
Branka Vuković-Gačić
; Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, Serbia
Draga Simić
; Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, Serbia
Jelena Knežević-Vukčević
; Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, Serbia
Sažetak
The use of dietary antimutagens and anticarcinogens has been seen as a promising approach to the protection of human health. Basil (Ocimum basilicum L.) is a well-known medicinal and aromatic plant, with a range of newly discovered biological activities possibly important for chemoprevention. In the preliminary experiments, toxic and mutagenic potential of essential oil (EO) from basil and pure substances: linalool, ß-myrcene and 1,8-cineole were tested using Salmonella typhimurium TA98, TA100 and TA102, with and without S9 mix (microsomal fraction of rat liver). No mutagenic effect of basil derivatives was detected in any tested strain. Antimutagenic effects of essential oil from basil and its pure constituents were further evaluated in the Ames test using S. typhimurium TA100. UVC irradiation and three chemical mutagens, 4-nitroquinoline-N-oxide (4NQO), 2-nitropropane (2-NP) and benzo(a)pyrene (B(a)P) were used to induce mutagenesis. All tested basil derivatives significantly reduced UV-induced mutations. The maximum inhibition was in the range of 64–77 %. Inhibitory potential against direct acting model mutagen/carcinogen 4NQO was similar to UV (52–67 %). In the presence of S9, EO and 1,8-cineole showed moderate inhibition of 2-NP induced mutagenesis, while the remaining two substances had no effect. Linalool exhibited high co-mutagenic effect with B(a)P, 1,8-cineole showed moderate inhibitory effect against B(a)P-induced mutations, while EO and ß-myrcene were ineffective.
Ključne riječi
4-nitroquinoline-N-oxide (4NQO); 2-nitropropane (2-NP); benzo(a)pyrene (B(a)P); UVC irradiation; Ocimum basilicum; Salmonella typhimurium TA100
Hrčak ID:
27781
URI
Datum izdavanja:
4.6.2007.
Posjeta: 3.346 *