Acta Adriatica, Vol. 64 No. 2, 2023.
Izvorni znanstveni članak
https://doi.org/10.32582/aa.64.2.3
Intraspecific characterization of Vibrio alginolyticus isolates recovered from aquaculture systems and marine biotopes in Tunisia by PCR-RFLP, ECP and OMPs profiling.
Rim Lajnef
orcid.org/0000-0002-2482-1052
; Laboratoire de Traitement des Eaux Usées, Centre de Recherches et des Technologies des eaux, Technopôle de Borj-Cédria, HammamLif, Tunisia
*
Lotfi Ben Abdallah
orcid.org/0000-0002-5084-0329
; Institut National des Sciences et Technologies de la Mer, Salammbô, Tunisia
Silvana Tapia-Paniagua
; Departamento de Microbiologia, Facultad de Ciencia de Malaga, Malaga, Spain
Yosra Turki
; Laboratoire de Traitement des Eaux Usées, Centre de Recherches et des Technologies des eaux, Technopôle de Borj-Cédria, HammamLif, Tunisia
Alberto Medina
; Departamento de Microbiologia, Facultad de Ciencia de Malaga, Malaga, Spain
Ines Mehri
; Laboratoire de Traitement des Eaux Usées, Centre de Recherches et des Technologies des eaux, Technopôle de Borj-Cédria, HammamLif, Tunisia
Miguel Angel Moriñigo
; Departamento de Microbiologia, Facultad de Ciencia de Malaga, Malaga, Spain
Abdennaceur Hassen
; Laboratoire de Traitement des Eaux Usées, Centre de Recherches et des Technologies des eaux, Technopôle de Borj-Cédria, HammamLif, Tunisia
* Dopisni autor.
Sažetak
A total of 54 presumptive Vibrio alginolyticus strains isolated from a variety of Tunisian marine biotopes, such as seawater, sediment samples from some bathing areas, and aquaculture farms, were characterized and identified by several biochemical tests. DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, extracellular products (ECP) and outer membrane protein (OMP) profiling were used to evaluate their usefulness as a tool to investigate the Vibrio alginolyticus diversity within this complex group. Results showed that there is great heterogeneity in the diversity observed via the PCR-RFLP method related to the number of genotypes generated by the two enzymes SduI and FaqI tested. This heterogeneity was observed not only according to the origin (seawater, sediment, fish and bivalve aquaculture farms) but also within the same type of sample. The two other methods, ECP and OMP resulted in 32 and 26 profiles, respectively. The discriminatory index determined in this study highlighted the good ability of the PCR-RFLP method to discriminate similar V. alginolyticus strains.
Ključne riječi
Vibrio alginolyticus; extracellular products (ECP); Outer membrane protein (OMP); PCR-RFLP; Tunisia
Hrčak ID:
307510
URI
Datum izdavanja:
25.8.2023.
Posjeta: 841 *