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https://doi.org/10.37427/botcro-2025-012

Micropropagation and optimisation of in vitro production of the rare and threatened moss Entosthodon pulchellus (Funariaceae)

Marija V. Ćosić ; University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden, Takovska 43, RS-11000 Belgrade, Serbia
Djordje P. Božović ; University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden, Takovska 43, RS-11000 Belgrade, Serbia
Michael S. Ignatov ; Lomonosov Moscow State University, Faculty of Biology, Plant Ecology and Geography Department, Leninskie Gory Str. 1–12, Moscow 119234, Russia
Elena A. Ignatova ; Lomonosov Moscow State University, Faculty of Biology, Plant Ecology and Geography Department, Leninskie Gory Str. 1–12, Moscow 119234, Russia
Milorad M. Vujičić ; University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden, Takovska 43, RS-11000 Belgrade, Serbia; Centre of Plant Biotechnology and Conservation (CPBC), Takovska 43, RS-11000 Belgrade, Serbia
Aneta D. Sabovljević ; University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden, Takovska 43, RS-11000 Belgrade, Serbia; Centre of Plant Biotechnology and Conservation (CPBC), Takovska 43, RS-11000 Belgrade, Serbia
Marko S. Sabovljević ; University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden, Takovska 43, RS-11000 Belgrade, Serbia; Centre of Plant Biotechnology and Conservation (CPBC), Takovska 43, RS-11000 Belgrade, Serbia; Pavol Jozef Šafárik University in Košice, Faculty of Science, Institute of Biology and Ecology, Department of Botany, Mánesova 23, SK-040 01 Košice, Slovakia *

* Autor za dopisivanje.

Sažetak

The establishment of axenic cultures and the propagation of the rare moss Entosthodon pulchellus (H. Philip.) Brugués (Funariaceae), as well as the optimisation of its ex situ growth conditions and rapid biomass production were the goals of this study. The results obtained also provide insights into the developmental biology of this moss in in vitro conditions, particularly regarding basal media contents with or without supplements of selected plant growth regulators or sugars, i.e. its nutritional needs. The procedure for establishing axenic cultures of this rare and threatened moss species is described. The optimisation protocol of the growth and production conditions is elaborated. The spore germinability of dried herbarium samples remained rather high in the tested laboratory conditions. Suitability tests on different media types showed KNOP basal media to be the best fit for the rapid biomass production of both secondary protonemata and leafy gametophores, without the addition of sugars or plant growth regulators. However, sugar, namely sucrose, induced rapid and massive protonemal development and can be used when this developmental stage is needed.

Ključne riječi

bryophyte; conservation; development; ex situ; growth

Hrčak ID:

319689

URI

https://hrcak.srce.hr/319689

Datum izdavanja:

1.10.2025.

Posjeta: 0 *