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Plate method for counting proteolytic sulphide-producing bacteria

Božidar Stilinović ; University of Zagreb, Faculty of Science, Division of Biology, Rooseveltov trg 6, 10000 Zagreb, Croatia
Jasna Hrenović orcid.org/0000-0001-8513-3993 ; University of Zagreb, Faculty of Science, Division of Biology, Rooseveltov trg 6, 10000 Zagreb, Croatia

Sažetak

The proteolytic sulphide-producing bacteria (PSPB) are widely distributed in water and sediment and are a good indicator of the ecological status of ecosystems. From the ecological point of view it is important to distinguish the physiological group of PSPB from other bacteria producing H2S from sulphate, sulphite or thiosulphate. A new medium named peptone-cysteine-ammonium iron citrate agar (PCA) was developed and tested. The medium regularly gave a higher number of colony forming units than the control media for detection of sulphide formation, indole production and motility (Merck) and iron sulphite agar (Oxoid). For the enumeration of PSPB from the environment, the simultaneous incubation of samples in aerobic and anaerobic conditions is recommended and a higher number should be taken for interpretation.

Ključne riječi

Isolation; bacteria; counting; sediment; sulphide; peptone; proteins

Hrčak ID:

36176

URI

https://hrcak.srce.hr/36176

Datum izdavanja:

30.4.2009.

Posjeta: 3.662 *