Izvorni znanstveni članak
A Novel Microbacterium sp. for Asymmetric Reduction of Simple Aromatic Ketone and Its Key Reductase
Li Luo
; College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, PR China
Zhong-Hua Yang
; College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, PR China
Rong Zeng
; College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China
Wei Zhou
; College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, PR China
Yan Zhao
; College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, PR China
Geng-Hua Chen
; College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, PR China
Hao Huang
; College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, PR China
Sažetak
A novel strain, Microbacterium sp., which can be used as a catalyst in the asymmetric reduction of simple aromatic ketones to produce chiral aromatic alcohols, was isolated from soil by microbial screening. Using acetophenone as a model substrate for asymmetric reduction, 82 % reaction yield with more than 99 % enantiomeric excess of (R)-1-phenylethanol were achieved under the optimal conditions. Furthermore, the key reductase responsible for the asymmetric reduction was purified from the newly isolated Microbacterium sp. through (NH4)2SO4 fractional precipitation, anion exchange chromatography and gel filtration chromatography. The purified reductase is a NADH-dependent aromatic ketone reductase. Gel filtration chromatography and SDS-PAGE results reveal that it is a homodimer with two 28-kDa subunits. The enzymatic properties of the reductase indicate that it is a highly efficient catalyst for the asymmetric reduction of simple aromatic ketone.
Ključne riječi
asymmetric reduction; Microbacterium sp.; aromatic ketone reductase; acetophenone
Hrčak ID:
109144
URI
Datum izdavanja:
9.9.2013.
Posjeta: 1.271 *