Izvorni znanstveni članak
https://doi.org/10.17113/ftb.53.01.15.3661
Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production
Nataša Bauer
; Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Rosemary Vuković
; Sveučilište u Osijeku, Osijek, Hrvatska
Saša Likić
; Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Sibila Jelaska
; Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Sažetak
Rosmarinic acid is one of the main active components of Coleus blumei and is known to have numerous health benefi ts. The pharmacological significance of rosmarinic acid and its production through in vitro culture has been the subject of numerous studies. Here, the ability of different tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. Calli, tumours, normal roots and hairy roots were established routinely by application of plant growth regulators or by transformation with agrobacteria. The differences among the established tumour lines were highly heterogeneous. Hairy root lines showed the highest mean growth rate and consistency in rosmarinic acid production. Although some tumour lines produced more rosmarinic acid than the hairy root lines, over a long cultivation period their productivity was unstable and decreased. Further, the effects of plant growth regulators on growth and rosmarinic acid accumulation were tested. 2,4-Dichlorophenoxyacetic acid significantly reduced tumour growth and rosmarinic acid production. 1-Naphthaleneacetic acid strongly stimulated hairy root growth whilst abscisic acid strongly enhanced rosmarinic acid production. Hairy roots cultured in an airlift bioreactor exhibited the highest potential for mass production of rosmarinic acid.
Ključne riječi
abscisic acid; callus; Coleus blumei; 2,4-dichlorophenoxyacetic acid; hairy root; 1-naphthaleneacetic acid and rosmarinic acid
Hrčak ID:
136918
URI
Datum izdavanja:
30.3.2015.
Posjeta: 3.092 *