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HPLC DEIERMINATION OF CATECEINS IN GRAPE SEEDS OF PLAVAC MALI CULTIVAR
Višnja Katalinić
; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska
Petar Maleš
; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska
Ljubica Šimac
; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska
Sažetak
Using HPLC technique on reversed-phase, the amounts of (+) - catechin and (-) epicatechin in grape seeds of autochthonous Dalmatian cultivar plavac mali, were analyzed during the growth season. To prevent interference between phenolic compounds, because many of them have similar UV absorption spectra, we fractionated acidic and neutral phenolic compounds on Sep Pak C 18 cartridges. Catechins were separated using HPLC with gradient solvent system at 280 mm. The grape seeds were analyzed during various stages of ripening. The results show the highest concentrations of catechins in the verison. The level decrease quickl troughout maturation. The major flavan- 3-ol monomer was (+) - catechin.
Ključne riječi
calauring stage; grape seeds; technological ripening stage
Hrčak ID:
142605
URI
Datum izdavanja:
21.3.1998.
Posjeta: 826 *