Izvorni znanstveni članak
https://doi.org/10.17113/ftb.55.04.17.5192
Threshold Level and Traceability of Roundup Ready® Soybeans in Tofu Production
Zorica Nikolić
; Institute of Field and Vegetable Crops, Maksima Gorkog 30, RS-21000 Novi Sad, Serbia
Gordana Petrović
; Institute of Field and Vegetable Crops, Maksima Gorkog 30, RS-21000 Novi Sad, Serbia
Dejana Panković
; Educons University, Vojvode putnika 85-87, RS-21208 Sremska Kamenica, Serbia
Maja Ignjatov
; Institute of Field and Vegetable Crops, Maksima Gorkog 30, RS-21000 Novi Sad, Serbia
Dragana Marinković
; Institute of Field and Vegetable Crops, Maksima Gorkog 30, RS-21000 Novi Sad, Serbia
Milan Stojanović
; Institute of Field and Vegetable Crops, Maksima Gorkog 30, RS-21000 Novi Sad, Serbia
Vuk Đorđević
; Institute of Field and Vegetable Crops, Maksima Gorkog 30, RS-21000 Novi Sad, Serbia
Sažetak
The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready® soybeans (RRS) at 0.9, 2, 3 and 5 % (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effective than conventional PCR in the analysis of raw soymilk, okara, boiled soymilk and tofu. The negative effect of grinding and mechanical manipulation was obvious in the okara sample prepared with 3 and 5 % RRS, where GMO content was reduced to (2.28±0.23) and (2.74±0.26) %, respectively. However, heating at 100 °C for 10 min did not cause significant degradation of DNA in all samples. The content of RRS in the final product, tofu, was reduced tenfold during processing, ranging from 0.07 to 0.46 %, which was below the labelling threshold level. The results are discussed in terms of global harmonization of GMO standards, which could have the positive effect on the trade of lightly processed foodstuffs such as tofu, especially regarding the labelling policies.
Ključne riječi
DNA degradation; GMO; labelling; legislation; tofu; traceability
Hrčak ID:
192317
URI
Datum izdavanja:
29.12.2017.
Posjeta: 1.264 *