Original scientific paper

Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase

Luigi Campanella ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Giulia Di Persio ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Manuela Pintore ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Daniele Tonnina ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Nicola Caretto ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Elisabetta Martini ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Dalina Lelo ; Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy

Abstract

A biosensor for rapid determination of nonsteroidal anti-inflammatory drugs (NSAIDs) is described based on the inhibition of cyclooxygenase enzyme (both isoforms) by NSAIDs. The results show the full validity of the method, which has also been optimized by comparing the inhibition of two enzyme isoforms, COX-1 and COX-2, in the presence of different tested pharmaceutical drugs (diclofenac, naproxen, ibuprofen, tolmetin). Also, recovery trials were performed in milk and fresh cheese adulterated with known quantities of NSAIDs, always obtaining recovery values >88 %.

Keywords

food analysis; COX; inhibition biosensor

Hrčak ID:

38111

URI

https://hrcak.srce.hr/38111

Publication date:

9.6.2009.

Article data in other languages: croatian

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