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Molecular method for identification of fallow venison

Tomislav Gomerčić ; Zavod za biologiju, Veterinarski fakultet Sveučilište u Zagrebu, Heinzelova 55, 10000 Zagreb.
Magda Sindičić ; Zavod za biologiju, Veterinarski fakultet Sveučilište u Zagrebu, Heinzelova 55, 10000 Zagreb.
Zdravko Janicki ; Zavod za biologiju, patologiju i uzgoj divljači, Veterinarski fakultet Sveučilište u Zagrebu, Heinzelova 55, 10000 Zagreb.
Tajma Trupec ; Veterinarski fakultet Sveučilište u Zagrebu, Heinzelova 55, 10000 Zagreb.
Ines Ines ; Veterinarski fakultet Sveučilište u Zagrebu, Heinzelova 55, 10000 Zagreb.
Dean Konjević orcid.org/0000-0001-6955-6419 ; Zavod za veterinarsku ekonomiku i epidemiologiju, Veterinarski fakultet Sveučilište u Zagrebu, Heinzelova 55, 10000 Zagreb.

Abstract

Reliable and simple identification of species origin of meat and meat products can be carried out by applying species-specific primers that
target particular short DNA fragments. The aim of our research was to design species-specific primers for the amplification of mitochondrial
DNA control region of fallow deer (Dama dama) and to optimize the polymerase chain reaction protocol (PCR protocol) for such primers. Primers are used for the identification of fallow deer and the differentiation of fallow deer from other species, using only DNA extraction, PCR and electrophoresis. The application of primers was tested on eight fallow deer samples, three red deer and three roe deer samples.

Keywords

primer; mitochondrial DNA; fallow deer; Dama dama

Hrčak ID:

149288

URI

https://hrcak.srce.hr/149288

Publication date:

1.6.2015.

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