Original scientific paper

https://doi.org/10.17113/ftb.56.04.18.5905

Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47

Yulian Tumbarski orcid.org/0000-0003-1674-5333 ; Department of Microbiology, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Ivelina Deseva orcid.org/0000-0003-0399-7507 ; Department of Analytical Chemistry and Physicochemistry, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Dasha Mihaylova orcid.org/0000-0002-6144-9640 ; Department of Biotechnology, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Magdalena Stoyanova ; Department of Analytical Chemistry and Physicochemistry, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Lutsian Krastev ; Department of Analytical Chemistry and Physicochemistry, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Radosveta Nikolova ; Department of Microbiology, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Velichka Yanakieva orcid.org/0000-0003-0862-6238 ; Department of Microbiology, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria
Ivan Ivanov orcid.org/0000-0002-6826-2723 ; Department of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, 26, Maritsa Blvd., 4002 Plovdiv, Bulgaria

Abstract

Members of the bacterial genus Bacillus are known as producers of a broad spectrum of antibiotic compounds of proteinaceous nature that possess inhibitory activity against different saprophytic and pathogenic microorganisms. In the current research, a peptide synthesized by Bacillus methylotrophicus strain BM47, previously isolated from a natural thermal spring in Bulgaria, was identified and characterized as a bacteriocin. In vitro antimicrobial screening of the crude bacteriocin substance of B. methylotrophicus BM47 showedn activity against the plant pathogenic fungi Fusarium moniliforme, Aspergillus awamori, Penicillium sp., Aspergillus niger and Gram-negative bacterium Pseudomonas aeruginosa. The antimicrobial activity of the crude bacteriocin substance was partially inhibited by the enzymes trypsin, Alcalase®, Savinase®, proteinase K, papain and Esperase®, while catalase was not effective. The crude bacteriocin substance was relatively pH resistant, but sensitive to the action of heat and most organic solvents and detergents tested. To obtain the active protein fractions, crude bacteriocin substance was purified by fast protein liquid chromatography (FPLC) using a strong anion exchange column. Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated that the purified bacteriocin had molecular mass of 19 578 Da. The amino acid analysis performed by high-performance liquid chromatography (HPLC) revealed that the isolated bacteriocin consisted of 17 types of amino acids, with the highest mol fraction expressed as percent of serine (29.3), valine (10.3), alanine (9.8) and tyrosine (7.1).

Keywords

Bacillus methylotrophicus; bacteriocin; antimicrobial activity; amino acid composition

Hrčak ID:

214960

URI

https://hrcak.srce.hr/214960

Publication date:

31.12.2018.

Article data in other languages: croatian

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