Review article

https://doi.org/10.31895/hcptbn.17.1-2.7

Plant protein hydrolysates as animal cell culture media supplement

Višnja Gaurina Srček ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Tino Ursić ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Marijan Logarušić ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Kristina Radošević ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Igor Slivac orcid.org/0000-0002-8344-1244 ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia

Abstract

Culture media containing essential nutrients like glucose, amino-acids and salts, as well as hormones, growth factors added by animal blood serum is required for bioprocesses with animal cells. Due to the disadvantages of serum use (e.g. risk of virus transmission, variation in composition, complex product isolation), the development of serum-free media supplemented with plant protein hydrolysates took place about two decades ago. The hydrolysates contain amino acids, oligopeptides, lipids, vitamins and other compounds that promote cell culture proliferation and productivity. This paper gives a brief overview of the preparation and the most important biological properties of protein hydrolysates with emphasis on the use of plant hydrolysates as components of animal cell culture media in the production of biopharmaceuticals.

Keywords

biopharmaceuticals, biopeptides, protein hydrolysates, productivity

Hrčak ID:

287129

URI

https://hrcak.srce.hr/287129

Publication date:

14.12.2022.

Article data in other languages: croatian

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