Original scientific paper
EFFECTIVENESS OF THE RAPID STRIP TESTS IN THE ANALYSIS OF THE GENETICALLY MODIFIED CORN’S PRESENCE IN FOOD PRODUCTS
Damir Alihodžić
; Agency for Halal Quality Certification, Turalibegova 73, 75000, Tuzla, Bosnia and Herzegovina
*
Midhat Jašić
; Faculty of Technology, University of Tuzla, Univerzitetska 8, 75000 Tuzla, Bosnia and Herzegovina
Benjamin Muhamedbegović
; Faculty of Technology, University of Tuzla, Univerzitetska 8, 75000 Tuzla, Bosnia and Herzegovina
Drago Šubarić
; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 18, 31000 Osijek, Croatia
Amel Selimović
; Faculty of Technology, University of Tuzla, Univerzitetska 8, 75000 Tuzla, Bosnia and Herzegovina
* Corresponding author.
Abstract
Food derived from genetically modified organisms is increasingly present in the diet of the world population. Genetic modification of soy and corn is especially frequent, resulting in products originating from these raw materials found in the market. Rapid and conventional methods may be used for monitoring and controlling the presence of genetically modified corn.
The aim of this study was to compare the use of rapid methods with conventional methods when analyzing ingredients derived from genetically modified corn.
In this study the presence of genetically modified corn has been analyzed in five products: polenta, muffin mixture and corn flakes from the USA, polenta from Norway and tortillas from Sweden. Conventional procedure based on polymerase chain reaction (PCR) and rapid method immunochromatography strip tests for CP4 ESPS protein detection were used to detect the ingredients originating from genetically modified corn. Sample preparation was done through homogenization and dissolved in polypropylene tube with distilled water in ratio 1:4. The results were read at nitrocellulose part of the strip test. For PCR method, the isolation of the DNA with application DNEasy Tissue Kit was done from prepared sample, followed by quantification of the isolated DNA with BioTek Powerwave HT Microplate spectrometer and application of Gen5 Data Analysis software. The PCR results were read with the application of electrophoresis with 1.5% of agar gel.
Both the rapid method and the conventional method showed similar results for all samples analysed. Polenta and muffin mixture originating from the USA contained genetically modified corn, whereas the products from the Nordic countries showed non-detectable levels.
Imunnochromatography strip tests are equally reliable as the PCR method in detection of genetically modified corn in food products. The rapid methods are several times cheaper, applicable outside the laboratory conditions and the analysis results are ready in 10-15 minutes.
Keywords
genetically modified corn; imunnochromatography strip test; PCR
Hrčak ID:
321349
URI
Publication date:
11.10.2024.
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