Izvorni znanstveni članak
Surfactant Supplementation to Enhance the Production of Vitamin K2 Metabolites in Shake Flask Cultures Using Escherichia sp. Mutant FM3-1709
Yan Liu
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Zhi-Ming Zheng
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Hong-Wei Qiu
; National Center for Biotechnology Development, 100036 Beijing, PR China
Gen-Hai Zhao
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Peng Wang
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Hui Liu
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Li Wang
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Zhe-Min Li
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
He-Fang Wu
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Hong-Xia Liu
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Mu Tan
; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Sažetak
The effects of the addition of various surfactants on the cell growth and production of vitamin K2 metabolites, such as intracellular menaquinone-4 (MK-4), extracellular MK-4, intracellular MK-6 and extracellular MK-6, were studied in the submerged culture of Escherichia sp. All of the added surfactants caused the extension of the exponential phase. Betaine, polyoxyethylene oleyl ether and Tween-80 were favourable to the cell growth of Escherichia sp. mutant strain FM3-1709. The highest cell growth (Xmax), biomass production rate (Qx) and biomass yield (Yx/s) were (12.6±0.2) g/L, (0.21±0.01) g/(L·h) and (2.42± 0.02) g/g, respectively. The results show that the addition of all surfactants led to a lower production of intracellular MK-4, whereas the production of extracellular MK-4 increased remarkably. Among the five investigated surfactants, the addition of the nonionic surfactant polyoxyethylene oleyl ether (1.0 g/L) led to the highest production of extracellular MK-4 ((33.6±0.4) mg/L), MK-6 ((2.56±0.07) mg/L) and the highest yield of total MK-4
((47.6±0.4) mg/L) and MK-6 ((6.0±0.1) mg/L). The addition of polyoxyethylene oleyl ether proved to be more beneficial for the secretion of MK-4 than MK-6.
Ključne riječi
surfactant; vitamin K2; shake flask cultures
Hrčak ID:
126170
URI
Datum izdavanja:
15.9.2014.
Posjeta: 1.903 *