Original scientific paper

Antimikrobna rezistencija koagulaza-negativnih stafilokoka iz tradicionalnih fermentiranih kobasica

Nevijo Zdolec orcid.org/0000-0001-7413-8115 ; University of Zagreb, Faculty of Veterinary Medicine, Department of Food Hygiene, Technology and Safety, Heinzelova 55, HR-10000 Zagreb, Croatia
Ivana Račić ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Anja Vujnović ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Maja Zdelar-Tuk ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Krešimir Matanović ; University of Zagreb, Faculty of Veterinary Medicine, Department of Microbiology and Infectious Diseases with Clinic, Heinzelova 55, HR-10000 Zagreb, Croatia
Ivana Filipović ; University of Zagreb, Faculty of Veterinary Medicine, Department of Food Hygiene, Technology and Safety, Heinzelova 55, HR-10000 Zagreb, Croatia
Vesna Dobranić ; University of Zagreb, Faculty of Veterinary Medicine, Department of Food Hygiene, Technology and Safety, Heinzelova 55, HR-10000 Zagreb, Croatia
Željko Cvetnić ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Silvio Špičić ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia

Abstract

U radu je primjenom klasičnih i molekularnih metoda istražena osjetljivost koagulaza-negativnih stafilokoka iz tradicionalnih fermentiranih kobasica na antimikrobne spojeve. Pomoću disk-difuzijskog i epsilometar testa ispitana je osjetljivost izolata na vankomicin, ampicilin, eritromicin, tetraciklin, gentamicin i oksacilin. Metodom je PCR u izolatima određena prisutnost gena za rezistenciju, i to mecA, ermB, tetK i tetM. Utvrđene su sljedeće vrste koagulaza-negativnih stafilokoka: Staphylococcus epidermidis (69 %), S. capitis (5 %) i S. warneri (2.5 %). Ukupno je 44,4 % izolata S. epidermidis pokazalo fenotipsku rezistenciju na tetraciklin i eritromicin. Prisutnost gena tetK ili tetM potvrđena je u 8 izolata S. epidermidis. Iako S. epidermidis nije uobičajeni uzročnik trovanja hranom, njegova je prisutnost u hrani važna za javno zdravstvo zbog mogućeg širenja antimikrobne rezistencije. Dobiveni rezultati pokazuju da spontanom fermentacijom mesa mogu nastati proizvodi opasni za zdravlje potrošača.

Keywords

fermentirane kobasice, Staphylococcus epidermidis, geni za rezistenciju

Hrčak ID:

104817

URI

https://hrcak.srce.hr/104817

Article data in other languages: english

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